Site-specific restriction endonucleases in cyanobacteria
نویسندگان
چکیده
منابع مشابه
Mesophilic cyanobacteria producing thermophilic restriction endonucleases.
When searching for the site-specific endonucleases in several strains of Phormidium we made the following observations. Among the 16 strains that originated from 15 species of Phormidium, 12 produced one or more restriction enzymes, of which two produced the highly thermophilic restriction endonucleases PtaI and PpaAII with their optimum activity at 65-80 degrees C, which is far above the letha...
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We present in Table I an updated list of the sensitivities of over 240 restriction endonucleases to the site-specific DNA modifications '14C, n-6C, h1"5C, and m6A, four modifications that are common in DNA prokaryotes, eukaryotes, and their viruses (Mc2,Mc5,Mc8,Mcl 1 ,Ne3,Ne4). Table II is a list of over 130 characterized DNA methyltransferases. A detailed list of cloned restrictionmodification...
متن کاملEffect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases
Restriction endonucleases have site-specific interactions with DNA that can often be inhibited by site-specific DNA methylation and other site-specific DNA modifications. However, such inhibition cannot generally be predicted. The empirically acquired data on these effects are tabulated for over 320 restriction endonucleases. In addition, a table of known site-specific DNA modification methyltr...
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Zinc-finger nucleases and TALE nucleases are produced by combining a specific DNA-binding module and a non-specific DNA-cleavage module, resulting in nucleases able to cleave DNA at a unique sequence. Here a new approach for creating highly specific nucleases was pursued by fusing a catalytically inactive variant of the homing endonuclease I-SceI, as DNA binding-module, to the type IIP restrict...
متن کاملCharacterization of the 5-hydroxymethylcytosine-specific DNA restriction endonucleases
In T4 bacteriophage, 5-hydroxymethylcytosine (5hmC) is incorporated into DNA during replication. In response, bacteria may have developed modification-dependent type IV restriction enzymes to defend the cell from T4-like infection. PvuRts1I was the first identified restriction enzyme to exhibit specificity toward hmC over 5-methylcytosine (5mC) and cytosine. By using PvuRts1I as the original me...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2000
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2000.01206.x